Isolation of streptococcal nuclease B by batch adsorption.

Simple method for the preparation of streptococcal nucleases.

A simple method for preparation and fractionation of the streptococcal nucleases by polyacrylamide gel electrophoresis is presented. The procedure is carried out with ammonium sulfate-precipitated supernatant fluids from cultures of beta-hemolytic streptococci grown to stationary phase. Electrophoresis on polyacrylamide gel and subsequent elution of the fractionated enzymes allows the preparati...

Streptococcal DNase B is immunologically identical to superantigen SpeF but involves separate domains.

The previous suggestion that streptococcal superantigen SpeF might be identical to DNase B was confirmed in this study. Polyclonal SpeF-specific antisera were able to inhibit depolymerization of methyl-green DNA by DNase B. However, T-cell mitogenicity and nuclease activity appear to involve separate immune epitopes on SpeF, since sera with the capacity to neutralize the mitogenic activity of S...

Adsorption of Volatile Organic Compounds from Aqueous Solution by Granular Activated Carbon (GAC) in Batch System

Batch Adsorption of Organic Dyes by Organo-bagasse: Carbon Content, pH Influence, Kinetics and Isotherms (RESEARCH NOTE)

The influence of carbon content in three organic dyes, blue21, yellow20, and green40 on the adsorption behavior using organo-bagasse as a biosorbent was investigated. The organo-bagasse was prepared by adsolubilisation using tetradecyltrimethyl ammonium bromide as a chemical modifying agent. The precursor and organo-bagasse were characterized by elemental analysis, Brunauer-Emmett-Teller method...

Cellular immune responses to extracellular streptococcal products in rheumatic heart disease.

The lymphocyte transformation responses to purified preparations of two extracellular products of group A streptococci (blastogen A and nuclease B), to phytohemagglutinin, and to Candida albicans antigen were measured in tonsillar and peripheral blood lymphocytes from patients with rheumatic heart disease (RHD) and suitably matched nonrheumatic (control) subjects. The mean phytohemagglutinin do...